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KMID : 0985420220440010036
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Laboratory Medicine and Quality Assurance
2022 Volume.44 No. 1 p.36 ~ p.39
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Evaluation of the Reliability of Automated Colistin Susceptibility Assays of Pseudomonas aeruginosa
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Kim Young-Ah
Kim Jung-Lim
Son Young-Jun
Lee Sang-Sun
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Abstract
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Background: With the increase in multidrug-resistant (MDR) gram-negative rods, colistin treatment is increasingly required. Reliable susceptibility testing is important for managing infectious patients with MDR Pseudomonas aeruginosa. This study evaluated the reliability of an automated colistin susceptibility assay.
Methods: In total, 195 non-duplicated P. aeruginosa blood isolates were collected from nine sentinel hospitals in Korea in 2020. Colistin susceptibility with Sensititre KNIHCOL Trek diagnostic system (Thermo Fisher Scientific, England), a commercial broth microdilution (BMD), was compared with that of the gram-negative MIC panel type 44 card (Beckman Coulter, USA). Isolates with discrepant results were reconfirmed using AST-N225 (bioMerieux SA, France).
Results: Categorical agreement was 92.8%, and nine colistin-resistant isolates with BMD were colistin-intermediate with two automated colistin susceptibility assays.
Conclusions: The failure to detect colistin-resistant P. aeruginosa could not be safely excluded with an automated colistin susceptibility assay.
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KEYWORD
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Colistin, Automated susceptibility assay, Broth microdilution, Pseudomonas aeruginosa
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